Figure 2

Figure 2: i) Device operation showing lysis zone creation, mixing with assay reagents, capture of reaction mixture in a chamber with valves, and the concentration-enhanced mobility shift assay; ii) Single cell lysis while other cells are unaffected; iii) Measurement of protein kinase MK2 activity from single cells by a fluorimetric assay; iv) Measurement of protein kinase Akt activity in ~15 cells using a mobility shift assay.

Figure 2: i) Device operation showing lysis zone creation, mixing with assay reagents, capture of reaction mixture in a chamber with valves, and the concentration-enhanced mobility shift assay; ii) Single cell lysis while other cells are unaffected; iii) Measurement of protein kinase MK2 activity from single cells by a fluorimetric assay; iv) Measurement of protein kinase Akt activity in ~15 cells using a mobility shift assay.