Figure 2
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Figure 2: a) Electropherogram for optimized electrokinetic concentration and separation of IgE aptamer (5nM) and different concentrations of IgE: (1) 4.92pM; (2) 0.6 nM, and (3) 6.75 nM IgE. The complex band is labeled with an asterisk; b) Inset demonstrates detection of 5 pM IgE in buffer; c) Dose-response curve of anti-IgE aptamer with IgE spiked in buffer, error bars represent standard error from duplicate experiments; d) Linear relationship in the log-log plot is obtained at low concentrations of IgE. The measured LOD is 4.4 pM IgE.