Figure 2

Figure 2: (A) Schematic of microfluidic perfusion device and mESCs growing in a single chamber in self-renewing medium. (B) Growth analysis of perfusion cultures in differentiation medium (N2B27) supplemented with Fgf4, and soluble factors saturated medium (N2B27+CM). (C) Expression levels of self-renewal (left) and differentiation (right) markers of mESCs growing in self-renewal medium in the device shown in (A) versus mESCs grown in a standard culture dish.

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    Akintunde Akinwande Anantha Chandrakasan Brian Wardle Carlijn Mulder Carl Thompson Caroline Ross Charles Sodini Chih-Hao Chang CICS Clifton Fonstad Dana Weinstein Dimitri Antoniadis Duane Boning Eugene Fitzgerald Faraz Najafi Francesco Marsili George Barbastathis Hae-Seung Lee Harry Tuller Ichiro Masaki Jason Sussman Jeffrey Lang Jesus del Alamo Jing Kong Joel Dawson Joel Voldman Jongyoon Han Judy Hoyt Karen Gleason Karl Berggren Lan Wei Luca Daniel Luis Velásquez-García Marc Baldo Omar Mysore Philip Reusswig Rajeev Ram Sang-Gook Kim Sangeeta Bhatia Scott Manalis Sean Bishop Tomas Palacios Vitor Manfrinato Vladimir Bulovic Vladimir Stojanovic