przybyla_diffusiblesignaling_01

Figure 1: (A) Schematic of microfluidic perfusion device and mESCs growing in a single chamber. (B) Growth analysis of perfusion cultures in differentiation medium (N2B27) supplemented with Fgf4, and soluble factor-saturated medium (N2B27+CM). (C) Images of cells cultured under perfusion in the indicated conditions and corresponding growth analysis. (D) Fluorescent images of cells grown under perfusion in the indicated conditions and corresponding Sox1-GFP+ cell frequency assessed by flow cytometry. * = p

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    ahmed al-obeidi akintunde akinwande anantha chandrakasan brian wardle carl thompson caroline ross center for excitonics charles sodini clifton fonstad dana weinstein dimitri antoniadis duane boning eugene fitzgerald farnaz niroui frances hill gallium nitride gang chen graphene hae-seung lee harry tuller healthcare iii-v materials jeffrey lang jesús del alamo jing kong joel voldman jongyoon han judy l. hoyt karl berggren lionel kimerling luca daniel luis velasquez-garcia marc baldo mildred dresselhaus pablo jarillo-herrero pao lin patrick brown qing hu sang-gook kim silvija gradecak tomas palacios vladimir bulovic vladimir stojanovic yu-chung hsiao zohaib mahmood