[labnetwork] Dilution of Triton X-100

Fri Aug 10 17:06:38 EDT 2012

We purchase both, BHF with and without surfactant pre-mixed in it.

Cheers!

Julia Aebersold, Ph.D.
MNTC Cleanroom Manager
Shumaker Research Building, Room 233
2210 South Brook Street
University of Louisville
Louisville, KY  40292

502-852-1572
http://louisville.edu/micronano/

From: labnetwork-bounces at mtl.mit.edu [mailto:labnetwork-bounces at mtl.mit.edu] On Behalf Of Morrison, Richard H., Jr.
Sent: Thursday, August 09, 2012 11:10 AM
To: Fouad Karouta; labnetwork at mtl.mit.edu
Subject: Re: [labnetwork] Dilution of Triton X-100

Here at Draper we dilute it with water, or if you are adding it to the BHF then you want 1% of the total volume to be TritonX-100, just premix it in water and then add that. It takes some time to get the Triton X-100 to mix into the water.

Why not just buy the BHF with a surfactant already mixed in. Transene Company can do this for you.

Rick

Rick Morrison
Senior Member Technical  Staff
Group Leader Microfabrication Operations
Draper Laboratory
555 Technology Square
Cambridge, MA  02139

W  617-258-3420
C   508-930-3461

From: labnetwork-bounces at mtl.mit.edu<mailto:labnetwork-bounces at mtl.mit.edu> [mailto:labnetwork-bounces at mtl.mit.edu]<mailto:[mailto:labnetwork-bounces at mtl.mit.edu]> On Behalf Of Fouad Karouta
Sent: Thursday, August 09, 2012 3:33 AM
To: labnetwork at mtl.mit.edu<mailto:labnetwork at mtl.mit.edu>
Subject: [labnetwork] Dilution of Triton X-100

Hello all,

Recently when discussing with Bill Flounders about adding a surfactant to BHF solution he suggested to use Triton X-100.
We like to use it in a diluted form. From internet I could find water, toluene, xylene and ethanol can be used as diluent. However water may turn the solution gelatinous.
Does anyone have experience with diluting triton x-100 (supplier Sigma Aldrich)?

Thanks,
Fouad Karouta

*********************************
Facility Manager ANFF ACT Node
Research School of Physics and Engineering
Australian National University
ACT 0200, Canberra, Australia
Tel: + 61 2 6125 7174
Mob: + 61 451 046 412
Email: fouad.karouta at anu.edu.au<mailto:fouad.karouta at anu.edu.au>
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